Publications
Research Articles
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Lampson, B. L.*, Ramίrez, A. S.*, Baro, M., He, L., Hegde, M., Koduri, V., Pfaff, J. L., Hanna, R. E., Kowal, J., Shirole, N. H., He, Y., Doench, J. G., Contessa, J. N., Locher, K. P., and Kaelin, W. G. (2024) Positive selection CRISPR screens reveal a druggable pocket in an oligosaccharyltransferase required for inflammatory signaling to NF-κB. Cell. 187, 2209-2223.e16. Link to the Article
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de Capitani, M. M., Ramírez, A. S., Rossi, L., Alexander, J. A. N., De Lorenzo, S., Locher, K. P., and Reymond, J.-L. (2023) Synthesis and characterisation of fluorescent substrates for eukaryotic protein N-glycosylation. Tetrahedron. 136, 133361. Link to the Article
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Ramírez, A. S., de Capitani, M., Pesciullesi, G., Kowal, J., Bloch, J. S., Irobalieva, R. N., Reymond, J.-L., Aebi, M., and Locher, K. P. (2022) Molecular basis for glycan recognition and reaction priming of eukaryotic oligosaccharyltransferase. Nat. Commun. 13, 7296. Link to the Article
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Bloch, J. S., Sequeira, J. M., Ramírez, A. S., Quadros, E. V., and Locher, K. P. (2022) Generation of nanobodies targeting the human, transcobalamin‐mediated vitamin B 12 uptake route. FASEB J. 36, e22222. Link to the Article
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Ramírez, A. S.*, Kowal, J*., and Locher, K. P. (2019) Cryo-electron microscopy structures of human oligosaccharyltransferase complexes OST-A and OST-B. Science. 366, 1372–1375. Link to the Article
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Ramírez, A. S., Boilevin, J., Mehdipour, A. R., Hummer, G., Darbre, T., Reymond, J. L., and Locher, K. P. (2018) Structural basis of the molecular ruler mechanism of a bacterial glycosyltransferase. Nat. Commun. 9, 445. Link to the Article
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Ramírez, A. S., Boilevin, J., Lin, C.-W., Ha Gan, B., Janser, D., Aebi, M., Darbre, T., Reymond, J.-L., and Locher, K. P. (2017) Chemo-enzymatic synthesis of lipid-linked GlcNAc2Man5 oligosaccharides using recombinant Alg1, Alg2 and Alg11 proteins. Glycobiology. 27, 726–733. Link to the Article
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Ramírez, A. S.*, Boilevin, J.*, Biswas, R., Gan, B. H., Janser, D., Aebi, M., Darbre, T., Reymond, J.-L., and Locher, K. P. (2017) Characterization of the single-subunit oligosaccharyltransferase STT3A from Trypanosoma brucei using synthetic peptides and lipid-linked oligosaccharide analogs. Glycobiology. 27, 525–535. Link to the Article
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Sloan, K. E., Leisegang, M. S., Doebele, C., Ramírez, A. S., Simm, S., Safferthal, C., Kretschmer, J., Schorge, T., Markoutsa, S., Haag, S., Karas, M., Ebersberger, I., Schleiff, E., Watkins, N. J., and Bohnsack, M. T. (2015) The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21. Nucleic Acids Res. 43, 553–564. Link to the Article
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Rodríguez, J. E., Ramírez, A. S., Salas, L. P., Helguera-Repetto, C., Gonzalez-y-Merchand, J., Soto, C. Y., and Hernández-Pando, R. (2013) Transcription of Genes Involved in Sulfolipid and Polyacyltrehalose Biosynthesis of Mycobacterium tuberculosis in Experimental Latent Tuberculosis Infection. PLoS One. 8, e58378. Link to the Article
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Rodríguez, J., Ramírez, A. S., Suárez, M. F., and Soto, C. Y. (2012) Electrochemical monitoring of the metabolic activity of mycobacteria in culture. Antonie van Leeuwenhoek, Int. J. Gen. Mol. Microbiol. 102, 193–201. Link to the Article
Book Chapters and Reviews
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Ramírez, A. S., and Locher, K. P. (2023) Structural and mechanistic studies of the N-glycosylation machinery: From lipid-linked oligosaccharide biosynthesis to glycan transfer. Glycobiology. 10.1093/GLYCOB/CWAD053. Link to the Article
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Ramírez, A. S.*, Nosol, K.*, and Locher, K. P. (2022) Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies. Methods Mol. Biol. 2507, 273–294. Link to the Chapter
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Leisegang, M. S., Martin, R., Ramírez, A. S., and Bohnsack, M. T. (2012) Exportin T and Exportin 5: tRNA and miRNA biogenesis-and beyond. Biol. Chem. 393, 599–604. Link to the Article